Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Analysis of immunohistochemical staining of RAGE and HMGB1 for TETs.

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Expression of HMGB1 in thymoma type A (A), B-component type AB (B), B1 (C) is shown. Scale bar: 40 μm. On this example of B1 thymoma lymphocytes are intermingled with few tumor cells. Focus on cytoplasmic staining: a larger magnification of a WHO type B1 thymoma is shown to better display “autophagic” tumor cells (tumor cells with brownish granular cytoplasmic and absent [only hematoxylin blue] nuclear staining). Scale bar: 20 μm (D). Analogously, a type B2 (E) and B3 (F) thymoma are shown. Two examples of TC (SCC (G) and (H)) are displayed – scale bar: 40 μm. HMGB1 high mobility group box1, TETs thymic epithelial tumors, TC thymic carcinoma, SCC squamous cell carcinoma

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: Expressing, Staining

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Immunohistochemistry revealed strong expression of RAGE in subcapsular cTEC of fetal (A) and adult thymus (B). Scale bar: 40 μm. For comparison the staining pattern of cytokeratins 5 and 14 – markers of epithelial cell origin on fetal thymus is shown (C). The expression pattern for HMGB1 in cTEC of fetal (D, scale bar: 40 μm) and adult thymus (E, scale bar: 20 μm) is shown. (F) A larger magnification of E is shown. Scale bar 8 μm. Arrows in F indicate HMGB1 cytoplasmic staining in cTEC. RAGE receptor for advanced glycation endproducts, HMGB1 high mobility group box1, cTEC cortical thymic epithelial cells

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: Immunohistochemistry, Expressing, Comparison, Staining

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Hassall's corpuscles stained with antibodies to RAGE (A), and HMGB1 (B, small arrows point to small GC) are displayed. Scale bar: 80 μm. Similarly, RAGE (C), and HMGB1 (D) staining in GC of MG patients (scale bar: 80 μm); and RAGE expression in thymic medulla (E, arrows point to thymic medulla; scale bar: 200 μm) and macrophages (F, scale bar: 40 μm) of regular adult thymus are shown. RAGE receptor for advanced glycation endproducts, HMGB1 high mobility group box1, MG Myasthenia gravis, GC germinal center.

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: Staining, Expressing

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Concentration of sRAGE, esRAGE and HMGB1 in serum of patients.

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: Concentration Assay

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Levels of sRAGE (a), esRAGE (b) and HMGB1 (c) in sera of patients with TETs including patients with paraneoplastic MG (MG n = 11) compared to healthy volunteers are shown. To rule out the influence of MG on levels of circulating sRAGE (d) and HMGB1 (e) in patients with TETs, patients with MG were excluded from this analysis. The levels of sRAGE in non-invasive (Masaoka-Koga stage I) and invasive TETs (Masaoka-Koga stages II-IV) are shown (f). RAGE receptor for advanced glycation endproducts, sRAGE soluble RAGE, esRAGE endogenous secretory RAGE, HMGB1 high mobility group box1, TETs thymic epithelial tumors, n number, MG Myasthenia gravis.

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: The levels of circulating sRAGE (a), and HMGB1 (b) in serum of patients with TETs compared to patients with thymic hyperplasia and healthy volunteers are shown. RAGE receptor for advanced glycation endproducts, sRAGE soluble RAGE, HMGB1 high mobility group box1, TET thymic epithelial tumor.

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques:

Journal: PLoS ONE

Article Title: Expression of RAGE and HMGB1 in Thymic Epithelial Tumors, Thymic Hyperplasia and Regular Thymic Morphology

doi: 10.1371/journal.pone.0094118

Figure Lengend Snippet: Patients with TETs were separated into patients with thymomas and TC, and compared to healthy volunteers. Serum concentrations of sRAGE (a) and HMGB1 (b) are shown. Patients with TC were analyzed compared to volunteers for sRAGE (c), esRAGE (d), as well as HMGB1 (e). TETs thymic epithelial tumors, RAGE receptor for advanced glycation endproducts, sRAGE soluble RAGE, esRAGE endogenous secretory RAGE, HMGB1 high mobility group box1, TC thymic carcinoma

Article Snippet: Sections were stained using affinity-purified polyclonal goat anti-human RAGE IgG (R&D Systems, Minneapolis, MN, USA) or monoclonal mouse anti-human HMGB1 IgG 2b (R&D Systems) and biotinylated anti-goat IgG or anti-mouse IgG secondary antibodies (Vector Laboratories, Burlingame, CA, USA).

Techniques: